Backbone Oligo Modification by Type
November 22, 2024 / December 20, 2024 by Dawn Lam
What backbone chemistries have been used for stereopure oligonucleotides? Traditional oligonucleotide synthesis using standard phosphoramidite methods does not control stereochemistry, resulting in a stereorandom phosphorothioate (PS) mixture where each linkage may adopt either R or S configuration. Studies in retinal disorders, CNS diseases, and muscular atrophy have demonstrated that stereopure oligonucleotides can significantly impact biological […]
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Oligo Modification By Application Spatial Transcriptomics
October 11, 2024 / December 20, 2024 by Dawn Lam
What is Spatial Transcriptomics? Spatial transcriptomics is a cutting-edge technology that allows researchers to study gene expression profiles of cells while maintaining their location within a tissue. This method has become popular in fields like oncology, neurobiology, and developmental biology due to its ability to map gene expression at single-cell resolution. By preserving tissue architecture, […]
5' Terminal Caps Oligo Modification by Type
September 18, 2024 / December 20, 2024 by Dawn Lam
What are 5′ triphosphate oligonucleotides and how are they being used? 5′ triphosphate (5′-PPP) oligonucleotides are short strands of nucleic acids with a triphosphate group attached to their 5′ end (Image 1). This 5′-triphosphate plays crucial roles in biological processes, particularly in RNA biology. Incorporating 5′-PPP into RNA or DNA oligos enables a variety of […]
Oligo Modification By Property Quality Control
July 10, 2024 / December 20, 2024 by Dawn Lam
What are endotoxins? Endotoxins, also known as lipopolysaccharides (LPS), are toxins produced by Gram-negative bacteria. They are released when the bacteria are killed or undergo cell lysis. LPS consists of three parts: lipid A, core polysaccharide, and O antigen. Lipid A is the toxic component responsible for triggering inflammatory and immune responses in mammals. Symptoms can vary […]
CRISPR Oligo Modification By Application
June 5, 2024 / December 24, 2024 by Dawn Lam
What is CRISPR Technology? CRISPR/CasX Technology (Clustered Regularly Interspaced Short Palindromic Repeats), derived from bacteria, has been a cornerstone of genetic engineering in the therapeutic industry for decades. The technology is versatile with its application, spanning across various fields, from agriculture to medicine, and enabling scientists to modify DNA sequences with remarkable accuracy. The most […]
Oligo Modification by Type Sugar
May 6, 2024 / December 20, 2024 by Dawn Lam
Why is Threose Nucleic Acid (TNA) modification being used? The use of oligonucleotides in therapeutics and diagnostics is rapidly expanding, particularly in the realm of antisense therapy. In previous discussions, we explored how modifying the backbone of antisense oligonucleotides (ASOs) with Mesyl can enhance stability and therapeutic efficacy. In this blog, we shift our focus to a […]
Check out our updated section below! What are antisense oligonucleotides (ASOs)? Antisense oligonucleotides (ASOs) are short nucleic acids designed to bind to specific RNA sequences, thereby inhibiting, reducing, restoring, or modifying the expression of target RNA. Over the past two decades, this method of gene silencing at the posttranscriptional stage has become increasingly popular as […]
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