biodistribution Archives - Synoligo

ASO and siRNA Biodistribution: Current Ligand Binding Assays

August 31, 2026 by Dawn Lam

Introduction Nucleic Acid Therapeutics (NATs), including antisense oligonucleotides (ASOs), splice-switching oligos, siRNAs and their conjugates act inside cells in tissue, not in plasma, so interpreting their pharmacology depends on measuring tissue concentrations of the intact drug in the presence of chain-shortened metabolites. This review covers the hybridization assay formats in current use, the chemistry that […]

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biodistribution siRNA ASO ligand binding assay hybridization ELISA electrochemiluminescence (ECL)Splint-ligation PCR

Oligo Modification By Property

Using Stable Isotope Labeling with ³⁴S for Pharmacokinetic and Biodistribution Studies

November 6, 2025 by Dawn Lam

If you go to https://clinicaltrials.gov/ and search “oligonucleotide” as the treatment, you’ll come up with over 260 trials which covers a variety of types of therapeutics modalities – ASOs, siRNAs, sgRNAs, and aptamers-most of which contain phosphorothioate linkages in the backbone. The reason for this is two-fold. First, the phosphorothioate linkages (PS) substantially increase nuclease […]

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isotope labeling biodistribution pharmacokinetic phosphorothioate