Oligo Modification By Property
June 18, 2026 / June 19, 2026 by Dawn Lam
Introduction to Antisense Oligos (ASOs) Antisense Oligonucleotides (ASOs) are fairly short oligonucleotides, typically 15-30 nucleotides in length, which are composed of either DNA, RNA or a mixture of both. The RNA nucleotides are invariably ribonucleoside analogs that lack a 2’-hydroxyl but still favor a 3’-endo sugar pucker such that the ASO-target duplex adopts a tighter, […]
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Oligo Modification By Application
May 4, 2026 / May 5, 2026 by Dawn Lam
Circular oligonucleotides are an emerging class of nucleic acid therapeutics (NATs) because of their resistance to exonucleases and their reduced immunogenicity.[1] Wesselhoeft et al., demonstrated that exogenous circRNA did not activate Toll-like Receptors (TLRs) nor Retinoic acid-Inducible Gene I (RIG-I), provided that the circRNA was meticulously purified to remove all linear RNA fragments. In addition, […]
Oligo Modification By Property Stability
January 4, 2025 / February 11, 2025 by Dawn Lam
What are some current nuclease resistance modifications that are being used? Oligonucleotides are susceptible to enzymatic degradation by exonucleases and endonucleases, particularly RNA, which is prone to both chemical instability and rapid breakdown. These challenges can significantly shorten their half-life and limit their effectiveness. To counteract these issues, various chemical modifications can be incorporated to improve […]
