Therapeutic Oligos
Custom Oligo Synthesis Coupled With High-Throughput In-Vitro Screening.
Overview
With the rise of targeted therapies powered by antisense, siRNA, and CRISPR technologies, oligonucleotides have become essential components in advancing modern medicine. At Synoligo, we specialize in delivering high-quality, custom oligos designed to meet the unique needs of clients across these cutting-edge therapeutic fields. From early-stage concepts to preclinical development, our team is committed to supporting the evolving demands of the biotech industry with precision, reliability, and deep expertise.
Stages We Cover
Explore our comprehensive solutions tailored for the development, manufacturing and testing of oligonucleotides from research to preclinical stage.
Gene Editing
CRISPR/sgRNA: cas9, cas12a
pegRNA: prime editing up to 200mer
RNA Editing
Gene Expression/ Upregulation or Silencing
ASO fully modified or conjugated
PMO: peptide or antibody conjugated
siRNA/miRNA/saRNA: click & maleimide chemistry ready
Protein Expression
mRNA, self-amplifying RNA, circular mRNA
Molecular Targeting
Aptamer:
DNA-, RNA, TNA-, Spiegelmer-based, PEGylated, bispecific
Hepatic and Extrahepatic Delivery
XOC:
Peptide, protein, antibody, small molecule conjugated
Other Oligos
Immunostimulatory agents:
CpG1018, CpG7909, 5’-di- and 5’-triphosphate
What We Offer
Our services can support your therapeutic program from early discovery to lead oligo selection and optimization!
Find Your Lead Candidates Faster With Us!
Overview
With our robust and streamlined workflows, we can rapidly synthesize and screen oligonucleotide libraries to identify leads with optimal activity, minimal toxicity, and strong target engagement, enabling faster, data-driven development decisions.
Why a One-Stop Shop Matters:
End-to-End Support
Fewer Handoffs for a
Seamless Process
Quick Data for Go/No-Go
Decisions
Fastest Path to Lead
Optimization
High-Throughput & Scalable Synthesis of Modified Oligos
Cell-Based Screening for Potency and Selectivity
In-Vitro Assessment of Cell-Based Toxicity and Immune Response
High-Throughput & Scalable Synthesis of Modified Oligos
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Deliver large quantities of modified oligos within 3 weeks:
- Up to 5,000 modified oligos at 1 nmol scale.
- Up to 200 modified oligos for mouse studies.
- Up to 100 modified oligos for non-GLP NHP studies.
- Delivered in 96 or 384-well plates at your desired concentration for cell screening.
Advanced Modification Expertise (see our full mod list)
- Rare and common 2′-position ribosugar modifications.
- Various backbone phosphoryl-linkages.
Custom Conjugation
- On-resin or post-synthesis conjugation of lipids, carbohydrates, peptides, and more.
Cell-Based Screening for Potency and Selectivity
- Rapidly screen > 240 oligos/week in a wide range of cell models by qRT-PCR.
- Customized luciferase reporter assay for ON/OFF-target screening.
- Provide high-throughput nucleic acid extraction and purification ready for sequencing and downstream selectivity analysis.
In-Vitro Assessment of Toxicity and Immune Response
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Ability to measure cell death and toxicity using:
- Cell titer/glo.
- LDH release assays.
- Caspase 3/7 assays.
- Screening with specific pathway dependent reporter cell models.
- Screening for cytokine response in primary human PBMCs.
Case Studies
Potency Screening:
On-Target Knockdown of ASOs and siRNAs
- The RT-qPCR assay has been validated for PCR efficiency and tested in both 1-Plex and 2-Plex formats (data not shown). Results demonstrated a strong dose-response knockdown for ASO and GalNAc-siRNA molecules in Hep3B and HepG2 cells.
- The luciferase reporter assay has been established and optimized to detect dose-response knockdown of siRNA at 48 hours post-transfection.
RT-qPCR Assay
Luciferase Reporter Assay
Advantages
- Flexible plasmid design and production capabilities.
- Utilize luciferase plasmids to express full-length or partial transcripts.
- High-throughput workflow allows testing multiple gene targets in the same cell line.
Applications
- Ideal for targets with low expression in standard cell lines.
- Ideal for screening specific sequences (e.g. mismatches) or ranking sequences and chemical modifications.
- Ideal for ON/OFF target screening conducted in parallel.
Applications of Oligos
With decades of experience, our expert team specializes in manufacturing oligos tailored to diverse scientific applications, ensuring your specific needs are met.
Research Oligos
Extensive list of customization allows researchers to achieve their research goals.
- Branched/dendrimer
- Circular DNA/RNA
- Ultra-long oligos (150–200nt)
- DNA-encoded library (DEL)
Diagnostic Oligos
Broad selection of various dyes and probes.
- Primers PCR/qPCR
- FISH/MERFISH Probes
- FRET Probes
- NGS Adapters
- Molecular Beacons
Therapeutic Oligos
Guarantee ultra low endotoxin even for lipid modified oligo.
- Antisense
- Immunostimulatory
- mi-/sa-/siRNA
- Aptamer
- CRISPR gRNA
