Backbone Oligo Modification by Type
November 22, 2024 / February 10, 2025 by Dawn Lam
What backbone chemistries have been used for stereopure oligonucleotides? Traditional oligonucleotide synthesis using standard phosphoramidite methods does not control stereochemistry, resulting in a stereorandom phosphorothioate (PS) mixture where each linkage may adopt either R or S configuration. Studies in retinal disorders, CNS diseases, and muscular atrophy have demonstrated that stereopure oligonucleotides can significantly impact biological […]
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5' Terminal Caps Oligo Modification by Type
September 18, 2024 / February 19, 2025 by Dawn Lam
What are 5′ triphosphate oligonucleotides and how are they being used? 5′ triphosphate (5′-PPP) oligonucleotides are short strands of nucleic acids with a triphosphate group attached to their 5′ end (Image 1). This 5′-triphosphate plays crucial roles in biological processes, particularly in RNA biology. Incorporating 5′-PPP into RNA or DNA oligos enables a variety of […]
Oligo Modification by Type Sugar
May 6, 2024 / February 19, 2025 by Dawn Lam
Why is Threose Nucleic Acid (TNA) modification being used? The use of oligonucleotides in therapeutics and diagnostics is rapidly expanding, particularly in the realm of antisense therapy. In previous discussions, we explored how modifying the backbone of antisense oligonucleotides (ASOs) with Mesyl can enhance stability and therapeutic efficacy. In this blog, we shift our focus to a […]
May 6, 2024 / March 3, 2025 by Dawn Lam
Check out our updated section below! What are antisense oligonucleotides (ASOs)? Antisense oligonucleotides (ASOs) are short nucleic acids designed to bind to specific RNA sequences, thereby inhibiting, reducing, restoring, or modifying the expression of target RNA. Over the past two decades, this method of gene silencing at the posttranscriptional stage has become increasingly popular as […]
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