a) Spin Down and Resuspend: Before uncapping, spin the sample down and vortex thoroughly with formulation buffer until the solution is clear. b) UV-Vis Dilution: For standard UV-Vis, prepare serial dilutions to achieve a reading between 0.1 and 1. Avoid exceeding a 200-fold dilution at any step. c) NanoDrop Optimization: If using a NanoDrop, ensure your dilution results in a reading between 5 and 10 for optimal accuracy.

The only reliable parameter is the absorbance at 260 nm (A260 or OD), which corresponds to the Optical Density per mL (i.e. the absorbance at 260nm normalized to a 1mL pathlength) for concentration calculations. Do not use the µg/µL value from the NanoDrop, as its conversion factor is based on the average extinction coefficients of standard DNA bases, not modified oligos.

µmol = (OD of single strand x1000)/EC mg= (µmol x MW)/1000

We recommend using a Nanodrop One or a more advanced model for more accurate results. To ensure high accuracy and reproducibility, run triplicates and prepare appropriate dilutions so the final A260 reading is between 5-10 to avoid saturation. High absorbance can underestimate the true concentration. For Nanodrop 8000, we recommend a dilution factor of 150-200X and the A260 reading should be ≈ 5.

The variation could be caused by inaccurate dosage/concentration, or sub-optimal formulation (e.g., lack of cation optimization, which can lead to acute toxicity for CNS studies). We recommend resuspending the whole vial to avoid moisture, sodium and analytical balance limitation from the extra weighing/aliquoting step.

Yes, you can send us >100µL of your dosing solution. We will validate both the concentration and osmolality for you.

Yes, our method is internally validated. We routinely check calibration curves, sensitivity, and accuracy with reference standards to ensure consistent, reliable readouts.

Yes. Synoligo can produce ultra-long DNA or RNA oligos, with or without modifications, up to 180–250 bases using chemical synthesis. For sequences that exceed this range, we can extend the length limitation through an enzymatic method.

Yes. This service is available through our custom oligo synthesis. To get started, please complete the contact form or email us at oligosales@synoligo.com, and our team will be glad to assist you.

Yes, we can. And in fact, we have most likely already done so for other customers. Our core strength lies in customization, and we offer one of the industry’s most comprehensive ranges of modification options. If a modification is chemically viable, we can incorporate it into an oligo. To ensure quality, we partner with leading vendors to source premium raw materials.

Yes, challenge accepted. Our scientists thrive on tackling complex projects. We frequently collaborate with customers who were turned away by other CROs because their requests were considered too difficult or incompatible with standard platform technologies. At Synoligo, we specialize in finding solutions where others cannot.

Yes. We routinely deliver lipid-conjugated oligos with endotoxin levels below 0.005 EU/mg. In addition, we can assist in removing endotoxin contamination from oligo products using our proprietary purification processes.

The cost depends on several factors, including scale, length, modifications, and purification requirements. For detailed pricing, please contact us.

Regardless of oligo length, we guarantee delivery of the quantity you request.

Depending on the complexity of the modification(s) or purification(s) requested, custom oligos will take at least 15 business days for production and shipping. Expedited service with 2-10 business days is available per request. Please check your order confirmation for your specific turnaround time.

Custom DNA or RNA oligos usually come in dry format. However, we can also customize to your specific formulation needs.

Primers resuspended in TE or water at ≥ 100µM are stable at -20°C up to 6 months. Dry oligos are stable at -20° for up to 1 year. * Frequent freeze/thaw will reduce shelf life and stability.