Oligo Modification By Application

Altering Splicing by Targeting a Steric Blocking Oligo to a Splice Junction of a pre-mRNA

September 13, 2025 / September 13, 2025 by Dawn Lam

Targeting a steric-blocking oligo to a splice junction usually causes a predictable change in splicing, though unexpected splice outcomes occur for some targets. Exons, except the first or last, are called “internal” exons; different splice outcomes are expected when blocking internal versus first-or-last exon splice junction targets. Here we show two examples of targeting internal […]

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